You are trying to clone YFG (your favorite gene) into pBR328

You are trying to clone YFG (your favorite gene) into pBR328

You are trying to clone YFG (your favorite gene) into pBR328 (note, this is not the one on the slide… Show more You are trying to clone YFG (your favorite gene) into pBR328 (note, this is not the one on the slide in class, Google is your friend). A) What restriction enzymes could you use? B) What would your SELECTION for plasmid transformation be? D) Would this plasmid make white or blue cells in E. coli strain DH5alpha? What origin(S) of replication are present on that plasmid? E) is this plasmid mobilizable? • Show less

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